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apotransferrin human  (Merck KGaA)

 
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    Structured Review

    Merck KGaA apotransferrin human
    Apotransferrin Human, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apotransferrin+human/apotransferrin+human/10__7554_slash_elife__87083__3-261-29-32
    Average 90 stars, based on 1 article reviews
    apotransferrin human - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Red Blood Cell Lysis:

    Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.87083 17 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Goat polyclonal anti- mouse Olig2 Biotechne Cat#AF2418, RRID:AB_2157554 1:200 Antibody Mouse monoclonal anti- mouse NeuN (clone A60) Merck Millipore Cat#MAB377, RRID:AB_2298772 1:100 Antibody Alexa Fluor 594_ donkey polyclonal antiMouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21203, RRID:AB_141633 1:400 Antibody Alexa Fluor 647_ donkey polyclonal anti- mouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21202A- 31571, RRID:AB_162542 1:400 Antibody Alexa Fluor 647_ donkey polyclonal antimouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A32787, RRID:AB_2762830 1:400 Chemical compound, drug Percoll Merck Millipore Cat#9048- 46- 8 22% Chemical compound, drug Poly- Lornithine solution Merck Millipore P4957 Chemical compound, drug Poly- Dlysine solution Merck Millipore 25988- 63- 0 Chemical compound, drug NeuroCult Basal medium STEMCELL Technologies Cat#05700 Chemical compound, drug Heparin Solution STEMCELL Technologies Cat#07980 Chemical compound, drug Epidermal Growth Factor Protein, human recombinant Merck Millipore Cat#GF144 Chemical compound, drug Fibroblast growth Factor basic, human recombinant Merck Millipore Cat#GF003AF Chemical compound, drug DMEM:F12 medium Thermo Fisher Scientific 31331028 Chemical compound, drug B- 27- Supplement Minus Antioyxidants (AO) Thermo Fisher Scientific Cat#10889038 Chemical compound, drug Fetal Bovine serum Thermo Fisher Scientific Cat#10082139 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Chemical compound, drug Tamoxifen Sigma- Aldrich Cat#T5648 Chemical compound, drug D-(+)Glucose Merck Millipore Cat#G7021 Chemical compound, drug Sodium bicarbonate Merck Millipore Cat#S5761 Chemical compound, drug HEPES sodium salt Merck Millipore Cat#H3784 Continued on next page Continued Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 18 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Insulin from bovine pancreas Merck Millipore Cat#I1882 Chemical compound, drug ApoTransferrin human Merck Millipore CatT2252 Chemical compound, drug Progesterone Merck Millipore Cat#P6149 Chemical compound, drug Putrescine Merck Millipore Cat#P7505 Chemical compound, drug Sodium selenite Merck Millipore Cat#S9133 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Commercial assay or kit Red blood Cell lysis solution Milteny Biotec Cat#130- 094- 183 Commercial assay or kit Dead Cell Removal Kit Milteny Biotec Cat#130- 090- 101 Commercial assay or kit RNeasy Micro Kit QIAGEN Cat#74004 Commercial assay or kit RNase- Free DNase Set QIAGEN Cat#79254 Commercial assay or kit Agilent High Sensitivity DNA Kit LabChip Cat#5067–4626 Commercial assay or kit Chromium Next GEM Chip G Single Cell Kit 10 X GENOMICS Cat#PN- 1000127 Commercial assay or kit Dual Index Kit Plate TT Set A 10 X GENOMICS Cat#PN- 1000215 Software, algorithm RStudio RStudio https://www.rstudio.com/ Software, algorithm Transcriptome Analysis Console (TAC 4.0) Thermo Fisher Scientific Software, algorithm limma Ritchie et al., 2015; Smyth et al., 2015 Software, algorithm EventPointer Romero et al., 2016a; Romero et al., 2016b Other DAPI stain Sigma- Aldrich D1306 Materials and methods >Immunolabeling 1 μg/ml Other Hoechst 33258 Thermo Fisher Scientific Cat#H3569 Materials and methods> Cell sorting 1 μg/ml Other Propidium iodide Sigma- Aldrich Cat#P4170 Materials and methods> Cell sorting 1 μg/ml Other Corn oil Sigma- Aldrich Cat#C8267 Tamoxifen solution 40 mg/ml Other Solution de paraformaldéhyde, 4% en PBS Thermo Fisher Scientific Cat#15670799 Materials and methods > Tissue processing Continued Continued on next page Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 19 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Triton X- 100 Sigma- Aldrich Cat# 11332481001 Materials and methods >Immunolabeling 0.1% Other 70 μm cell strainer Thermo Fisher Scientific Cat#10788201 Materials and methods > Single cell library preparation Other 2.5 μl, Model 62 RN SYR, Small Removable NDL, 22 s ga, 2 in, point style 3 Hamilton Cat#87942 Materials and methods > Transplants Other Small Hub 33 G Hamilton Cat#074750 Materials and methods > Transplants Continued

    Single Cell:

    Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.87083 17 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Goat polyclonal anti- mouse Olig2 Biotechne Cat#AF2418, RRID:AB_2157554 1:200 Antibody Mouse monoclonal anti- mouse NeuN (clone A60) Merck Millipore Cat#MAB377, RRID:AB_2298772 1:100 Antibody Alexa Fluor 594_ donkey polyclonal antiMouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21203, RRID:AB_141633 1:400 Antibody Alexa Fluor 647_ donkey polyclonal anti- mouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21202A- 31571, RRID:AB_162542 1:400 Antibody Alexa Fluor 647_ donkey polyclonal antimouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A32787, RRID:AB_2762830 1:400 Chemical compound, drug Percoll Merck Millipore Cat#9048- 46- 8 22% Chemical compound, drug Poly- Lornithine solution Merck Millipore P4957 Chemical compound, drug Poly- Dlysine solution Merck Millipore 25988- 63- 0 Chemical compound, drug NeuroCult Basal medium STEMCELL Technologies Cat#05700 Chemical compound, drug Heparin Solution STEMCELL Technologies Cat#07980 Chemical compound, drug Epidermal Growth Factor Protein, human recombinant Merck Millipore Cat#GF144 Chemical compound, drug Fibroblast growth Factor basic, human recombinant Merck Millipore Cat#GF003AF Chemical compound, drug DMEM:F12 medium Thermo Fisher Scientific 31331028 Chemical compound, drug B- 27- Supplement Minus Antioyxidants (AO) Thermo Fisher Scientific Cat#10889038 Chemical compound, drug Fetal Bovine serum Thermo Fisher Scientific Cat#10082139 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Chemical compound, drug Tamoxifen Sigma- Aldrich Cat#T5648 Chemical compound, drug D-(+)Glucose Merck Millipore Cat#G7021 Chemical compound, drug Sodium bicarbonate Merck Millipore Cat#S5761 Chemical compound, drug HEPES sodium salt Merck Millipore Cat#H3784 Continued on next page Continued Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 18 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Insulin from bovine pancreas Merck Millipore Cat#I1882 Chemical compound, drug ApoTransferrin human Merck Millipore CatT2252 Chemical compound, drug Progesterone Merck Millipore Cat#P6149 Chemical compound, drug Putrescine Merck Millipore Cat#P7505 Chemical compound, drug Sodium selenite Merck Millipore Cat#S9133 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Commercial assay or kit Red blood Cell lysis solution Milteny Biotec Cat#130- 094- 183 Commercial assay or kit Dead Cell Removal Kit Milteny Biotec Cat#130- 090- 101 Commercial assay or kit RNeasy Micro Kit QIAGEN Cat#74004 Commercial assay or kit RNase- Free DNase Set QIAGEN Cat#79254 Commercial assay or kit Agilent High Sensitivity DNA Kit LabChip Cat#5067–4626 Commercial assay or kit Chromium Next GEM Chip G Single Cell Kit 10 X GENOMICS Cat#PN- 1000127 Commercial assay or kit Dual Index Kit Plate TT Set A 10 X GENOMICS Cat#PN- 1000215 Software, algorithm RStudio RStudio https://www.rstudio.com/ Software, algorithm Transcriptome Analysis Console (TAC 4.0) Thermo Fisher Scientific Software, algorithm limma Ritchie et al., 2015; Smyth et al., 2015 Software, algorithm EventPointer Romero et al., 2016a; Romero et al., 2016b Other DAPI stain Sigma- Aldrich D1306 Materials and methods >Immunolabeling 1 μg/ml Other Hoechst 33258 Thermo Fisher Scientific Cat#H3569 Materials and methods> Cell sorting 1 μg/ml Other Propidium iodide Sigma- Aldrich Cat#P4170 Materials and methods> Cell sorting 1 μg/ml Other Corn oil Sigma- Aldrich Cat#C8267 Tamoxifen solution 40 mg/ml Other Solution de paraformaldéhyde, 4% en PBS Thermo Fisher Scientific Cat#15670799 Materials and methods > Tissue processing Continued Continued on next page Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 19 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Triton X- 100 Sigma- Aldrich Cat# 11332481001 Materials and methods >Immunolabeling 0.1% Other 70 μm cell strainer Thermo Fisher Scientific Cat#10788201 Materials and methods > Single cell library preparation Other 2.5 μl, Model 62 RN SYR, Small Removable NDL, 22 s ga, 2 in, point style 3 Hamilton Cat#87942 Materials and methods > Transplants Other Small Hub 33 G Hamilton Cat#074750 Materials and methods > Transplants Continued

    Software:

    Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.87083 17 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Goat polyclonal anti- mouse Olig2 Biotechne Cat#AF2418, RRID:AB_2157554 1:200 Antibody Mouse monoclonal anti- mouse NeuN (clone A60) Merck Millipore Cat#MAB377, RRID:AB_2298772 1:100 Antibody Alexa Fluor 594_ donkey polyclonal antiMouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21203, RRID:AB_141633 1:400 Antibody Alexa Fluor 647_ donkey polyclonal anti- mouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21202A- 31571, RRID:AB_162542 1:400 Antibody Alexa Fluor 647_ donkey polyclonal antimouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A32787, RRID:AB_2762830 1:400 Chemical compound, drug Percoll Merck Millipore Cat#9048- 46- 8 22% Chemical compound, drug Poly- Lornithine solution Merck Millipore P4957 Chemical compound, drug Poly- Dlysine solution Merck Millipore 25988- 63- 0 Chemical compound, drug NeuroCult Basal medium STEMCELL Technologies Cat#05700 Chemical compound, drug Heparin Solution STEMCELL Technologies Cat#07980 Chemical compound, drug Epidermal Growth Factor Protein, human recombinant Merck Millipore Cat#GF144 Chemical compound, drug Fibroblast growth Factor basic, human recombinant Merck Millipore Cat#GF003AF Chemical compound, drug DMEM:F12 medium Thermo Fisher Scientific 31331028 Chemical compound, drug B- 27- Supplement Minus Antioyxidants (AO) Thermo Fisher Scientific Cat#10889038 Chemical compound, drug Fetal Bovine serum Thermo Fisher Scientific Cat#10082139 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Chemical compound, drug Tamoxifen Sigma- Aldrich Cat#T5648 Chemical compound, drug D-(+)Glucose Merck Millipore Cat#G7021 Chemical compound, drug Sodium bicarbonate Merck Millipore Cat#S5761 Chemical compound, drug HEPES sodium salt Merck Millipore Cat#H3784 Continued on next page Continued Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 18 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Insulin from bovine pancreas Merck Millipore Cat#I1882 Chemical compound, drug ApoTransferrin human Merck Millipore CatT2252 Chemical compound, drug Progesterone Merck Millipore Cat#P6149 Chemical compound, drug Putrescine Merck Millipore Cat#P7505 Chemical compound, drug Sodium selenite Merck Millipore Cat#S9133 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Commercial assay or kit Red blood Cell lysis solution Milteny Biotec Cat#130- 094- 183 Commercial assay or kit Dead Cell Removal Kit Milteny Biotec Cat#130- 090- 101 Commercial assay or kit RNeasy Micro Kit QIAGEN Cat#74004 Commercial assay or kit RNase- Free DNase Set QIAGEN Cat#79254 Commercial assay or kit Agilent High Sensitivity DNA Kit LabChip Cat#5067–4626 Commercial assay or kit Chromium Next GEM Chip G Single Cell Kit 10 X GENOMICS Cat#PN- 1000127 Commercial assay or kit Dual Index Kit Plate TT Set A 10 X GENOMICS Cat#PN- 1000215 Software, algorithm RStudio RStudio https://www.rstudio.com/ Software, algorithm Transcriptome Analysis Console (TAC 4.0) Thermo Fisher Scientific Software, algorithm limma Ritchie et al., 2015; Smyth et al., 2015 Software, algorithm EventPointer Romero et al., 2016a; Romero et al., 2016b Other DAPI stain Sigma- Aldrich D1306 Materials and methods >Immunolabeling 1 μg/ml Other Hoechst 33258 Thermo Fisher Scientific Cat#H3569 Materials and methods> Cell sorting 1 μg/ml Other Propidium iodide Sigma- Aldrich Cat#P4170 Materials and methods> Cell sorting 1 μg/ml Other Corn oil Sigma- Aldrich Cat#C8267 Tamoxifen solution 40 mg/ml Other Solution de paraformaldéhyde, 4% en PBS Thermo Fisher Scientific Cat#15670799 Materials and methods > Tissue processing Continued Continued on next page Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 19 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Triton X- 100 Sigma- Aldrich Cat# 11332481001 Materials and methods >Immunolabeling 0.1% Other 70 μm cell strainer Thermo Fisher Scientific Cat#10788201 Materials and methods > Single cell library preparation Other 2.5 μl, Model 62 RN SYR, Small Removable NDL, 22 s ga, 2 in, point style 3 Hamilton Cat#87942 Materials and methods > Transplants Other Small Hub 33 G Hamilton Cat#074750 Materials and methods > Transplants Continued

    Staining:

    Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.87083 17 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Goat polyclonal anti- mouse Olig2 Biotechne Cat#AF2418, RRID:AB_2157554 1:200 Antibody Mouse monoclonal anti- mouse NeuN (clone A60) Merck Millipore Cat#MAB377, RRID:AB_2298772 1:100 Antibody Alexa Fluor 594_ donkey polyclonal antiMouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21203, RRID:AB_141633 1:400 Antibody Alexa Fluor 647_ donkey polyclonal anti- mouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21202A- 31571, RRID:AB_162542 1:400 Antibody Alexa Fluor 647_ donkey polyclonal antimouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A32787, RRID:AB_2762830 1:400 Chemical compound, drug Percoll Merck Millipore Cat#9048- 46- 8 22% Chemical compound, drug Poly- Lornithine solution Merck Millipore P4957 Chemical compound, drug Poly- Dlysine solution Merck Millipore 25988- 63- 0 Chemical compound, drug NeuroCult Basal medium STEMCELL Technologies Cat#05700 Chemical compound, drug Heparin Solution STEMCELL Technologies Cat#07980 Chemical compound, drug Epidermal Growth Factor Protein, human recombinant Merck Millipore Cat#GF144 Chemical compound, drug Fibroblast growth Factor basic, human recombinant Merck Millipore Cat#GF003AF Chemical compound, drug DMEM:F12 medium Thermo Fisher Scientific 31331028 Chemical compound, drug B- 27- Supplement Minus Antioyxidants (AO) Thermo Fisher Scientific Cat#10889038 Chemical compound, drug Fetal Bovine serum Thermo Fisher Scientific Cat#10082139 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Chemical compound, drug Tamoxifen Sigma- Aldrich Cat#T5648 Chemical compound, drug D-(+)Glucose Merck Millipore Cat#G7021 Chemical compound, drug Sodium bicarbonate Merck Millipore Cat#S5761 Chemical compound, drug HEPES sodium salt Merck Millipore Cat#H3784 Continued on next page Continued Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 18 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Insulin from bovine pancreas Merck Millipore Cat#I1882 Chemical compound, drug ApoTransferrin human Merck Millipore CatT2252 Chemical compound, drug Progesterone Merck Millipore Cat#P6149 Chemical compound, drug Putrescine Merck Millipore Cat#P7505 Chemical compound, drug Sodium selenite Merck Millipore Cat#S9133 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Commercial assay or kit Red blood Cell lysis solution Milteny Biotec Cat#130- 094- 183 Commercial assay or kit Dead Cell Removal Kit Milteny Biotec Cat#130- 090- 101 Commercial assay or kit RNeasy Micro Kit QIAGEN Cat#74004 Commercial assay or kit RNase- Free DNase Set QIAGEN Cat#79254 Commercial assay or kit Agilent High Sensitivity DNA Kit LabChip Cat#5067–4626 Commercial assay or kit Chromium Next GEM Chip G Single Cell Kit 10 X GENOMICS Cat#PN- 1000127 Commercial assay or kit Dual Index Kit Plate TT Set A 10 X GENOMICS Cat#PN- 1000215 Software, algorithm RStudio RStudio https://www.rstudio.com/ Software, algorithm Transcriptome Analysis Console (TAC 4.0) Thermo Fisher Scientific Software, algorithm limma Ritchie et al., 2015; Smyth et al., 2015 Software, algorithm EventPointer Romero et al., 2016a; Romero et al., 2016b Other DAPI stain Sigma- Aldrich D1306 Materials and methods >Immunolabeling 1 μg/ml Other Hoechst 33258 Thermo Fisher Scientific Cat#H3569 Materials and methods> Cell sorting 1 μg/ml Other Propidium iodide Sigma- Aldrich Cat#P4170 Materials and methods> Cell sorting 1 μg/ml Other Corn oil Sigma- Aldrich Cat#C8267 Tamoxifen solution 40 mg/ml Other Solution de paraformaldéhyde, 4% en PBS Thermo Fisher Scientific Cat#15670799 Materials and methods > Tissue processing Continued Continued on next page Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 19 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Triton X- 100 Sigma- Aldrich Cat# 11332481001 Materials and methods >Immunolabeling 0.1% Other 70 μm cell strainer Thermo Fisher Scientific Cat#10788201 Materials and methods > Single cell library preparation Other 2.5 μl, Model 62 RN SYR, Small Removable NDL, 22 s ga, 2 in, point style 3 Hamilton Cat#87942 Materials and methods > Transplants Other Small Hub 33 G Hamilton Cat#074750 Materials and methods > Transplants Continued

    Immunolabeling:

    Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.87083 17 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Goat polyclonal anti- mouse Olig2 Biotechne Cat#AF2418, RRID:AB_2157554 1:200 Antibody Mouse monoclonal anti- mouse NeuN (clone A60) Merck Millipore Cat#MAB377, RRID:AB_2298772 1:100 Antibody Alexa Fluor 594_ donkey polyclonal antiMouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21203, RRID:AB_141633 1:400 Antibody Alexa Fluor 647_ donkey polyclonal anti- mouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21202A- 31571, RRID:AB_162542 1:400 Antibody Alexa Fluor 647_ donkey polyclonal antimouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A32787, RRID:AB_2762830 1:400 Chemical compound, drug Percoll Merck Millipore Cat#9048- 46- 8 22% Chemical compound, drug Poly- Lornithine solution Merck Millipore P4957 Chemical compound, drug Poly- Dlysine solution Merck Millipore 25988- 63- 0 Chemical compound, drug NeuroCult Basal medium STEMCELL Technologies Cat#05700 Chemical compound, drug Heparin Solution STEMCELL Technologies Cat#07980 Chemical compound, drug Epidermal Growth Factor Protein, human recombinant Merck Millipore Cat#GF144 Chemical compound, drug Fibroblast growth Factor basic, human recombinant Merck Millipore Cat#GF003AF Chemical compound, drug DMEM:F12 medium Thermo Fisher Scientific 31331028 Chemical compound, drug B- 27- Supplement Minus Antioyxidants (AO) Thermo Fisher Scientific Cat#10889038 Chemical compound, drug Fetal Bovine serum Thermo Fisher Scientific Cat#10082139 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Chemical compound, drug Tamoxifen Sigma- Aldrich Cat#T5648 Chemical compound, drug D-(+)Glucose Merck Millipore Cat#G7021 Chemical compound, drug Sodium bicarbonate Merck Millipore Cat#S5761 Chemical compound, drug HEPES sodium salt Merck Millipore Cat#H3784 Continued on next page Continued Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 18 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Insulin from bovine pancreas Merck Millipore Cat#I1882 Chemical compound, drug ApoTransferrin human Merck Millipore CatT2252 Chemical compound, drug Progesterone Merck Millipore Cat#P6149 Chemical compound, drug Putrescine Merck Millipore Cat#P7505 Chemical compound, drug Sodium selenite Merck Millipore Cat#S9133 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Commercial assay or kit Red blood Cell lysis solution Milteny Biotec Cat#130- 094- 183 Commercial assay or kit Dead Cell Removal Kit Milteny Biotec Cat#130- 090- 101 Commercial assay or kit RNeasy Micro Kit QIAGEN Cat#74004 Commercial assay or kit RNase- Free DNase Set QIAGEN Cat#79254 Commercial assay or kit Agilent High Sensitivity DNA Kit LabChip Cat#5067–4626 Commercial assay or kit Chromium Next GEM Chip G Single Cell Kit 10 X GENOMICS Cat#PN- 1000127 Commercial assay or kit Dual Index Kit Plate TT Set A 10 X GENOMICS Cat#PN- 1000215 Software, algorithm RStudio RStudio https://www.rstudio.com/ Software, algorithm Transcriptome Analysis Console (TAC 4.0) Thermo Fisher Scientific Software, algorithm limma Ritchie et al., 2015; Smyth et al., 2015 Software, algorithm EventPointer Romero et al., 2016a; Romero et al., 2016b Other DAPI stain Sigma- Aldrich D1306 Materials and methods >Immunolabeling 1 μg/ml Other Hoechst 33258 Thermo Fisher Scientific Cat#H3569 Materials and methods> Cell sorting 1 μg/ml Other Propidium iodide Sigma- Aldrich Cat#P4170 Materials and methods> Cell sorting 1 μg/ml Other Corn oil Sigma- Aldrich Cat#C8267 Tamoxifen solution 40 mg/ml Other Solution de paraformaldéhyde, 4% en PBS Thermo Fisher Scientific Cat#15670799 Materials and methods > Tissue processing Continued Continued on next page Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 19 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Triton X- 100 Sigma- Aldrich Cat# 11332481001 Materials and methods >Immunolabeling 0.1% Other 70 μm cell strainer Thermo Fisher Scientific Cat#10788201 Materials and methods > Single cell library preparation Other 2.5 μl, Model 62 RN SYR, Small Removable NDL, 22 s ga, 2 in, point style 3 Hamilton Cat#87942 Materials and methods > Transplants Other Small Hub 33 G Hamilton Cat#074750 Materials and methods > Transplants Continued

    FACS:

    Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis
    Article Snippet: DOI: https://doi.org/10.7554/eLife.87083 17 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Goat polyclonal anti- mouse Olig2 Biotechne Cat#AF2418, RRID:AB_2157554 1:200 Antibody Mouse monoclonal anti- mouse NeuN (clone A60) Merck Millipore Cat#MAB377, RRID:AB_2298772 1:100 Antibody Alexa Fluor 594_ donkey polyclonal antiMouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21203, RRID:AB_141633 1:400 Antibody Alexa Fluor 647_ donkey polyclonal anti- mouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A21202A- 31571, RRID:AB_162542 1:400 Antibody Alexa Fluor 647_ donkey polyclonal antimouse IgG (H+L) Secondary Antibody Thermo Fisher Scientific Cat#A32787, RRID:AB_2762830 1:400 Chemical compound, drug Percoll Merck Millipore Cat#9048- 46- 8 22% Chemical compound, drug Poly- Lornithine solution Merck Millipore P4957 Chemical compound, drug Poly- Dlysine solution Merck Millipore 25988- 63- 0 Chemical compound, drug NeuroCult Basal medium STEMCELL Technologies Cat#05700 Chemical compound, drug Heparin Solution STEMCELL Technologies Cat#07980 Chemical compound, drug Epidermal Growth Factor Protein, human recombinant Merck Millipore Cat#GF144 Chemical compound, drug Fibroblast growth Factor basic, human recombinant Merck Millipore Cat#GF003AF Chemical compound, drug DMEM:F12 medium Thermo Fisher Scientific 31331028 Chemical compound, drug B- 27- Supplement Minus Antioyxidants (AO) Thermo Fisher Scientific Cat#10889038 Chemical compound, drug Fetal Bovine serum Thermo Fisher Scientific Cat#10082139 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Chemical compound, drug Tamoxifen Sigma- Aldrich Cat#T5648 Chemical compound, drug D-(+)Glucose Merck Millipore Cat#G7021 Chemical compound, drug Sodium bicarbonate Merck Millipore Cat#S5761 Chemical compound, drug HEPES sodium salt Merck Millipore Cat#H3784 Continued on next page Continued Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 18 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug Insulin from bovine pancreas Merck Millipore Cat#I1882 Chemical compound, drug ApoTransferrin human Merck Millipore CatT2252 Chemical compound, drug Progesterone Merck Millipore Cat#P6149 Chemical compound, drug Putrescine Merck Millipore Cat#P7505 Chemical compound, drug Sodium selenite Merck Millipore Cat#S9133 Chemical compound, drug Papain Worthington Biochemical Cat#LK003150 Commercial assay or kit Red blood Cell lysis solution Milteny Biotec Cat#130- 094- 183 Commercial assay or kit Dead Cell Removal Kit Milteny Biotec Cat#130- 090- 101 Commercial assay or kit RNeasy Micro Kit QIAGEN Cat#74004 Commercial assay or kit RNase- Free DNase Set QIAGEN Cat#79254 Commercial assay or kit Agilent High Sensitivity DNA Kit LabChip Cat#5067–4626 Commercial assay or kit Chromium Next GEM Chip G Single Cell Kit 10 X GENOMICS Cat#PN- 1000127 Commercial assay or kit Dual Index Kit Plate TT Set A 10 X GENOMICS Cat#PN- 1000215 Software, algorithm RStudio RStudio https://www.rstudio.com/ Software, algorithm Transcriptome Analysis Console (TAC 4.0) Thermo Fisher Scientific Software, algorithm limma Ritchie et al., 2015; Smyth et al., 2015 Software, algorithm EventPointer Romero et al., 2016a; Romero et al., 2016b Other DAPI stain Sigma- Aldrich D1306 Materials and methods >Immunolabeling 1 μg/ml Other Hoechst 33258 Thermo Fisher Scientific Cat#H3569 Materials and methods> Cell sorting 1 μg/ml Other Propidium iodide Sigma- Aldrich Cat#P4170 Materials and methods> Cell sorting 1 μg/ml Other Corn oil Sigma- Aldrich Cat#C8267 Tamoxifen solution 40 mg/ml Other Solution de paraformaldéhyde, 4% en PBS Thermo Fisher Scientific Cat#15670799 Materials and methods > Tissue processing Continued Continued on next page Bernou et al. eLife 2023;12:RP87083. .. DOI: https://doi.org/10.7554/eLife.87083 19 of 26 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Other Triton X- 100 Sigma- Aldrich Cat# 11332481001 Materials and methods >Immunolabeling 0.1% Other 70 μm cell strainer Thermo Fisher Scientific Cat#10788201 Materials and methods > Single cell library preparation Other 2.5 μl, Model 62 RN SYR, Small Removable NDL, 22 s ga, 2 in, point style 3 Hamilton Cat#87942 Materials and methods > Transplants Other Small Hub 33 G Hamilton Cat#074750 Materials and methods > Transplants Continued



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    apotransferrin human - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    human apotransferrin  (R&D Systems Hematology)
    90
    R&D Systems Hematology human apotransferrin
    Human Apotransferrin, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apotransferrin+human/human+apotransferrin/pm38580776-546-39-41
    Average 90 stars, based on 1 article reviews
    human apotransferrin - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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